Multiple determinations with moving-boundary electrophoresis.
نویسندگان
چکیده
OVING-BOUNDARY BLECTROPUORESIS is a widely used technic for the investigation of colloidal systems. A limitation of this method, however, is the considerable amount of time required for a single experiment. It is particularly time consuming where the accumulation of large amounts of data is necessary. Furthermore, in the majority of instruments only 1 experiment at a time, and usually a total of only 2-3 per day, can be performed. In a previous publication (1) an electrophoresis cell was described in which two determinations can be performed simultaneously. Using such dual-analysis cells, the authors have developed a procedure for multiple electrophoretic analysis which permits up to 12 such experiments to be performed in an 8-hour day.
منابع مشابه
Nonlinear vibration analysis of axially moving strings in thermal environment
In this study, nonlinear vibration of axially moving strings in thermal environment is investigated. The vibration haracteristics of the system such as natural frequencies, time domain response and stability states are studied at different temperatures. The velocity of the axial movement is assumed to be constant with minor harmonic variations. It is presumed that the system and the environment...
متن کاملResolution of fungal cellulase by zone electrophoresis.
Results of paper and column chromatography (1, 2) and paper electrophoresis (3) of crude preparations of fungal cellulase have pointed toward the multiple nature of this system. Results of ultracentrifugation and moving boundary electrophoresis of fractionated cellulase have, on the other hand, suggested its uniensymatic nature (4). In the present study, zone electrophoresis over extended dista...
متن کاملDetermination of stationary region boundary in multiple reference frames method in a mixing system agitated by Helical Ribbon Impeller using CFD
The multiple reference frames (MRF) method is the most suitable method tosimulate impeller rotation in mixing systems. Precise determination of stationaryand moving zones in MRF method leads to accurate results in mixing performance.In this research, the entire volume of mixing system was divided into two zones.The kinetic energy values were used to distinguish the zones with differentvelocitie...
متن کاملPolyacrylamide-gel electrophoresis of soybean whey proteins and trypsin inhibitors.
Polyacrylamide·.g~1 electrophoresis with glycine buffer (pH 9.2) containing 8 M urca separated soybean whey proteins into at least 24 bands. In contrast, ultracentrifugation indicated only 2 fractions; moving-boundary electrophoresis, 8-9 components; and column chromatography, 13 or more proteins. A prominent, fast-moving band in the gel pattern appeared to be identical to crystalline soybean t...
متن کاملAsymptotic Approximations of the Solution for a Traveling String under Boundary Damping
Transversal vibrations of an axially moving string under boundary damping are investigated. Mathematically, it represents a homogenous linear partial differential equation subject to nonhomogeneous boundary conditions. The string is moving with a relatively (low) constant speed, which is considered to be positive. The string is kept fixed at the first end, while the other end is tied with the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 2 5 شماره
صفحات -
تاریخ انتشار 1956